São Paulo School of Advanced Science on Biophysical Methods to Study Biomolecular Interactions |
The São Paulo Advanced School of Science on Biophysical Methods to Study Biomolecular Interactions, sediada no Instituto de Fisica da USP, será um marco nas Escolas de formação em Biofísica Molecular de caráter multi e interdisciplinar, sendo um evento inédito no país.
O objetivo da Escola é dar aos alunos uma visão abrangente do estado da arte de técnicas biofísicas experimentais e teóricas no estudo de interações em sistemas biomoleculares.
A programação está dividida em aulas teóricas com práticas experimentais ministradas por especialistas renomados em técnicas de fluorescência, SAXS, EPR, dicroísmo circular, AFM, NMR, DLS, Crio-microscopia e bioinformática.
Além das aulas teóricas, algumas palestras serão proferidas por conferencistas internacionais buscando mostrar um sinergismo entre as técnicas biofísicas na investigação dos mecanismos de ação envolvidos em interações biomoleculares em diferentes sistemas. Os resultados devem gerar impactos científicos e tecnológicos, além da formação abrangente e consolidada de novos recursos humanos.
O programa ainda contempla espaços em que os estudantes expõem e discutem seus projetos de pesquisa: 6 sessões de Casos de Estudo de 1 hora cada e 2 sessões de painéis de 2 horas cada. Com isto, esperamos propiciar um fórum de discussões e novas colaborações durante a Escola.
Finalmente, existem visitas a laboratórios de pesquisa: i) lab de Cristalografia (IFUSP) e de NMR (IQUSP); ii) visita programada de um dia para as facilidades do CNPEM, Campinas (LNLS, LNBio, LNNano).
A Advanced School on Biophysical Methods prevê a presença de 100 estudantes, sendo que 50 serão selecionados de outros paises e25 de outros estados do Brasil. Existe possibilidade de financiamento FAPESP para até 75 estudantes (incluindo passagem aérea e hospedagem).
Ressalta-se que os alunos interessados devem se inscrever através do site (www.fap.if.usp.br/~espcabio), encaminhando um CV resumido, carta de apoio do orientador/supervisor (no caso de pos-doc) indicando os benefícios esperados com a participação na Escola para sua formação e desenvolvimento de seu trabalho.
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Instituição
Instituto de Física/IF/USP
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Área de conhecimento
Fisiologia
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Pesquisador Responsável
Rosangela Itri
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Nº do Processo FAPESP
2016/18796-4
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Período
16/10/2017 a 27/10/2017
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Inscrições
Encerradas
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Site
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Cidade
São Paulo
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Palavras-chave
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Programa
Ocultar
October, 2017 – IF – USP
Program: First Week
Monday,
Oct 16th
Tuesday,
Oct 17th
Wednesday, Oct 18th
Thrusday,
Oct 19th
Friday,
Oct 20th
7h30-8h15
Registration
8h15
Opening
8h30
L1. Microscopy and Fluorescence Methodologies in Membrane Biophysics
Manuel Prieto
L5. Introduction to Cryo-Electron Microscopy, with key application examples
Jose Maria Carazo
L9 Biological and biomedical applications of atomic force microscopy
Nuno Santos
L11. Introduction to Biomolecular NMR
Christina Redfield
L12.Basic Computational approaches in biomolecular simulations: protein folding and protein assembly Yaakov Levy
10h00
Coffee Break
Coffee Break
Coffee Break
Coffee Break
Coffee Break
10h30
L2. Membrane SAXS: basic principles and applications
Rosangela Itri
L6. SAXS from proteins and protein-membrane interaction
Leandro Barbosa
L10. Proteins in membranes: lipid annular region, aggregation and topology by advanced fluorescence methodologies
Manuel Prieto
FAPESP time
Poster Session
12h00
Lunch with teachers
Lunch with teachers
Lunch with teachers
Lunch with teachers
Lunch w/ teachers
14h00
L3. Electron Spin Resonance: Theory and Biophysical Applications
Antonio Jose da Costa Filho
L7. Computing cryo-EM maps: The image processing workflow to go from 2D to 3D
Jose M. Carazo
Hands-on
I, II, III, IV and V
Hands-on
I, II, III, IV and V
Hands-on
II, III, IV, V
15h30
Coffee Break
Coffee Break
15h45
L4. AFM-based force spectroscopy
Nuno Santos
L8. Introduction to Basic Principles of NMR
Roberto Salinas
Hands-on
I, II, III, IV and V
Hands-on
I, II, III, IV and V
Hands-on
II, III, IV, V
17h15 – 18h15 *
Students Case Studies
L1-L4
Students Case Studies
L5-L8
Students Case Studies
L9-L10
Students Case Studies
L11
Students Case Studies
L12
Program: Second Week
Monday,
Oct 23rd
Tuesday,
Oct 24th
Wednesday, Oct 25th
Thrusday,
Oct 26th
Friday,
Oct 27th
8h15
8h30
L13. Molecular principles for optimizing protein-DNA interactions
Yaakov Levy
L15 Introduction to Molecular Dynamic Simulations of Membranes (Part I)
Kaline Coutinho
L17 CD spectroscopy: membrane proteins and drug interactions
Bonnie Wallace
Travel to Campinas (CNPEM)
Talk1. Synergy between NMR, cryo-EM and large- scale MD simulations – an all atom model of a native HIV capside
Angela M Gronenborn
10h00
Coffee Break
Coffee Break
Coffee Break
Coffee Break
Coffee Break
10h30
L14. Introduction to Assignment of Protein NMR Spectra
Christina Redfield
L16. CD spectroscopy: basic principles and applications to proteins (including peptides and IDPs)
Robert Janes
L18 The effect of post-translational modifications on protein biophysics
Yaakov Levi
Visit CNPEM - LNLS – LNBio-LNNano
Talk2. FRET images applied to living cells
Manuel Prieto
12h00
Lunch with teachers
Lunch with teachers
Lunch with teachers
Lunch at CNPEM
Lunch with teachers
14h00
Hands-on
III, IV, V and VI
Hands-on
III, IV, V and VI
L19 Introduction to Molecular Dynamic Simulations of Membranes (Part II)
Kaline Coutinho
Visit CNPEM - LNLS – LNBio-LNNano
Talk3 . The effect of post-translational modifications on protein biophysics
Yaakov Levi
15h30
Coffee Break
15h45
Hands-on
III, IV, V and VI
Hands-on
III, IV, V and VI
Poster Session
Visit CNPEM - LNLS – LNBio-LNNano
Talk4 . Exploiting Multiple Spectroscopies to quantify biological signaling in cells
Anthony Watts
17h15 – 18h15 *
Students Case Studies
L13-L14
Students Case Studies
L15-L16
Poster Session
Travel back to SP
Closing Ceremony : Awards
*Case Studies: some students pre-selected during the registration process (online registration) will present and discuss their results based on the lecturers (themes) given at each day.
*Lunch with teachers: students will be divided in small groups and have lunch with the teachers to exchange experiences and ideas.
Hands-On: the students will be split into 5 groups such that each group will attend a specific hands-on per afternoon (from I to V). Further, each student must choose, at least, 3 hands-on.
Hands-on I: 50 students in the auditorium: Hands-on in cryo Electron Microscopy image processing – Jose Maria Caraso (Wednesday October 18th and Thursday October 19th)
Electron Microscopy under cryogenic conditions (“cryo EM”) is changing the way we study the three dimensional structure of biological macromolecules, allowing to reach quasi-atomic resolution for flexible samples without the need to grow 3D crystals. The current “resolution revolution” has been made possible thanks to key technological developments in EM instrumentation and on new image processing breakthroughs. Focusing on the latter, at the Instruct Image Processing Center (I2PC) in Madrid (ALBA) we have developed a workflow oriented integrative image processing platform, that we call “Scipion”, that makes very intuitive all image processing steps. During this Demo we will remotely access the I2PC and run Scipion via Web, analyzing the most typical workflow for the analysis of biological macromolecules and some of its main steps, depending on the time allocated to this activity. Attendees will have the opportunity to log-in into a number of Demo accounts running Scipion from the attendee’s own computer through a web browser.
Hands-on II: 10 students in the Crystallography lab (IFUSP) and class room: Hands-on Characterization of colloidal systems based on laser Scattering techniques: DLS and NTA – Dra Lígia Nunes de Morais Ribeiro (IB-Unicamp)
Colloidal systems are heterogeneous and composed of a dispersed particulate phase in a non-miscible solution. They are widely applied in healthy, food and environmental fields, but they can be difficult to characterize due to their intrinsic dynamism. The light scattering approaches are the most employed techniques for evaluating the nanoparticles size distributions in colloids based on their Brownian motion. Dynamic light scattering (DLS) is the most used method for the measure of size distribution and Zeta potential by the intensity correlation function of the scattered light by the nanoparticles. Nanotracking analysis (NTA) is an innovator real-time technique that is changing the way to characterize nanoparticles in suspension. It comprises a laser source plus microscope (20x), charge-coupled device (CCD) camera and analytical software, providing individual measure of the dispersed nanoparticles. It also allows for the determination of the number of particles in suspension (concentration) by the “particle-by-particle” approach. Both techniques work in the 1-1000 nm range, at very diluted samples. In this hands-on, monodisperse and polydisperse samples will be measured, and the attendees will be trained to operate, understand and discuss the results obtained from both techniques, focusing in the suitable data quality parameters. Such information helps to understand the molecular interaction between their components: drug-carrier, RNA-exosome, and many others application, such as treatment monitoring and diagnosis.
Hands-on III: 20 students in a class room: EPR data analysis – Antonio Jose da Costa Filho (FFCLRP-USP)
EPR results from lipid model membranes containing spin-labeled lipids at different temperatures will be investigated. The molecular dynamics and ordering of the lipids will be analyzed as a function of the depth within the bilayer. The spectral analyses will be based on semi-empirical approaches as well as simulations using specific softwares.
Hands-on IV: 10 students in a Crystallography lab (IFUSP) and class room: SAXS of proteins and membranes: measurement and analysis – Rosangela Itri and Leandro Barbosa (IFUSP)
Samples of lipid model membranes in the presence of proteins will be measured at SAXS set-up (NanoStar) (IFUSP). The basic structural parameters as radius of gyration, shape and maximum dimension of the scattering objects will be retrieved from the SAXS curves as well as modelling using specific softwares will be done.
Hands-on V: 10 students in the NMR facility (IQUSP) and class room: NMR: measurement and data analysis – Roberto Salinas (IQUSP) and Eneida de Paula (IB-Unicamp)
Visit to the NMR facility; acquisition of 1D and 2D NMR spectra of proteins in water and small molecules; a short A short tutorial on the analysis of NMR titration to study protein-ligand interaction will be offered with the possibility for students to carry out data analysis using their own laptop computers. In this case students will be requested to install the specific NMR softwares in their laptops prior to the realization of the course. There will no time available for software installation.
Hands-on VI: 20 students in a classroom: CD spectroscopy on Data Processing and Methods of Analysis – Bonnie Wallace (Birkbeck College), Robert W James (Un. London) and Jose Luiz Lopes (IFUSP)
In this practice, students will remotely access the Protein CD Data Base (PCDDB) developed by profs. Bonnie Wallace and Robert W James and will perform a complete analysis of the secondary structure of an “unknown” folded protein and thermally unfolded.
Poster Session: there will be two posters sections dedicated to the students presentation.
Visit to CNPEM: the students will have the opportunity to know the facilities of the Brazilian National Lab: LNLS (in special the SAXS and IR beam lines), LNBIO (NMR facility) and LNNano (Cryo-TEM and negative stain microscopy)
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Notícias
Relacionadas Inscrições abertas para a Escola Avançada em Biofísica
Agência FAPESP, 15/05/2017
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