São Paulo School of Advanced Sciences on Neglected Diseases Drug Discovery – focus on Kinetoplastids

A SPSAS-ND3 foi realizada de 14 a 24 de junho de 2015 no campus do Centro Nacional de Pesquisa em Energia e Materiais (CNPEM), em Campinas (SP).

A Escola, organizada pelo Laboratório Nacional de Biociências (LNBio) e financiada pela FAPESP, contemplou aspectos multidisciplinares do processo de descoberta de fármacos aplicados à doença de Chagas, leishmaniose e à tripanossomíase africana humana.

A SPSAS-ND3 abordou desde conceitos básicos da ciência de descoberta de fármacos até as investigações de ponta desenvolvidas nesta área.

Dentre os grandes temas da programação, destacam-se: high throughput screening (HTS) e high content screening (HCS); biologia estrutural e screening virtual; análises de dados de triagens; química medicinal; otimização de leads; absorção, distribuição, metabolismo e excreção (ADME) in vitro e in vivo; e modelos animais para estudos de farmacocinética e estudos de eficácia.

  • Intituição

    Laboratório Nacional de Biociências / CNPEM / MCTI

  • Área de conhecimento

    Química

  • Pesquisador Responsável

    Lucio Holanda Gondim de Freitas Junior

  • Nº do Processo FAPESP

    2013/50644-4

  • Período

    14/06/2015 a 24/06/2015

  • Inscrições

    Encerradas

  • Site

    http://pages.cnpem.br/drugdiscovery-kinetoplastids

  • Cidade

    Campinas

  • Palavras-chave

    Fármacos, Doenças negligenciadas, Saúde, Química, Biociências

  • Programa Ocultar
    June, 14th

    13:00 –15:00: Lunch and Registration

    15:00 –16:00: Groups division: Organizing Committee

    16:00 – 6:30: Coffee-break

    16:30 –17:30: Welcoming and General Remarks
     
    June, 15th

    08:20 – 08:45: Overview of SPSAS-ND3: dynamics and expected outcomes
    Dr. Lucio Freitas-Junior – LNBio/ Brazil

    08:45 – 09:30: Neglected Tropical Diseases: Needs and Current Drug Discovery Landscape
    Dr. Eric Chatelain – DNDi/ Switzerland

    09:30 – 10:00: Drug Discovery: The Pharmaceutical Industry Perspective
    Dr. Sheraz Gul – ScreeningPort Fraunhofer/ Germany

    10:00 – 10:45: Introduction to Drug Discovery/Design of Biochemical Assays for Drug Discovery Purposes – what can be achieved and learnings from past success and failures
    Dr. Sheraz Gul – ScreeningPort Fraunhofer/ Germany

    10:45 – 11:15: Coffee-break

    11:15 – 12:00: Identification of metabolic targets in trypanosomatids
    Dr. Artur Cordeiro - LNBio/ Brazil

    12:00 – 12:45: Virtual Screening
    Dr. Rafael Guido – USP/ Brazil

    13:00 – 14:10: Lunch
     
    14:10 – 14:40: Poster fixation
     
    14:40 – 15:30: Biosafety in level II laboratory/ Instructions for practical activities
    Dr. Carolina Borsoi / Dr. Vanessa Fontana
     
    15:30 – 16:00: Official photography
     
    16:00 – 16:50: Coffee-break
     
    16:50 – 17:10: Opening ceremony
    Dr. Kleber Franchini - LNBio/ Brazil
     
    17:10 – 17:30: Opening ceremony
    Eric Stobbaerts - DNDi Latin America
     
    17:30 – 17:50: Inaugural Class
    Prof. Michel Rabinovitch – Unifesp/Brazil
     
    17:50-19:50: Poster session I/ Opening cocktail: Brazilian evening
    Supported by DNDi
     
    June, 16th

    08:30 – 09:15 Screening jargon and terms
    Dr. Sheraz Gul – ScreeningPort Fraunhofer/ Germany
     
    09:15 – 10:00 HTS Assay Design
    Dr. Sheraz Gul – ScreeningPort Fraunhofer/ Germany
     
    10:00 - 10:45 Biochemistry of Kinetoplastids and Drug Discovery
    Prof. Sergio Schenkman - UNIFESP/ Brazil
     
    10:45 – 11:15 Coffee-break
     
    11:15 – 12:00 Drug targets in Trypanosomes
    Prof. Joana Tavares – Universidade do Porto/ Portugal
     
    12:00 – 12:45 Current Chemotherapy for leishmaniasis: Drug Mechanism of Action
    Prof. Simon Croft - LSHTM/UK
     
    13:00 – 14:10 Lunch

    14:10 – 16:10 Practice I – Biochemical Assays

    Module 1 – Protease assay development and validation- Dr. Sheraz Gul – Group A and B – Room 09
    LNBio
    Module 2 – High throughput binding screening using MicroScale Thermophoresis - Nanotemper – Group
    C and D –Room 22 LNBio
    Module 3 – Using LabWare LIMS/ELN to catalogue Chemical Structures & ELN (Electronic Laboratory
    Notebook) Workshop - LabWare – Group E and F – Room 69 LNBio
    Module 4 – Fluorescence intensity assay for Trypanosoma brucei viability using CLARIOStar – Photonics/
    Dr. Bruno Pascoalino – Group G and H – Room 42 LNBio
     
    16:10 – 16:30 Coffee-break
     
    16:30 – 18:30 Practice I – Biochemical Assays
     
    Module 1 – Protease assay development and validation - Dr. Sheraz Gul – Group C and D - Room 09
    LNBio
    Module 2 – High throughput binding screening using MicroScale Thermophoresis - Nanotemper – Group
    E and F– Room 22 LNBio
    Module 3 – Using LabWare LIMS/ELN to catalogue Chemical Structures & ELN (Electronic Laboratory
    Notebook) Workshop - LabWare – Group G and H – Room 69 LNBio
    Module 4 – Fluorescence intensity assay for Trypanosoma brucei viability using CLARIOStar – Photonics/
    Dr. Bruno Pascoalino – Group A and B – Room 42 LNBio

    June, 17th

    08:30 – 09:15 The Path to a Candidate: A multidisciplinary Effort and a Lot of Hurdles
    Dr. Eric Chatelain - DNDi/ Switzerland
     
    09:15 – 10:00 High Throughput and High Content Screening for Trypanosomes
    Prof. Vicky M Avery - Eskitis Institute/Griffith University/ Australia
     
    10:00 - 10:45 MiRNA screening for drug discovery
    Dr. Musa Mhlanga – University of Cape Town & CSIR & IMM/ South Africa
     
    10:45 – 11:15 Coffee-break
     
    11:15 – 12:00 Integrative Drug Discovery Programs
    Dr. Sheraz Gul – ScreeningPort Fraunhofer/ Germany
     
    12:00 – 12:45 HCS Data Analysis and Reduction
    Dr. Kamyar Hadian - Helmholtz Zentrum München/ Germany
     
    13:00 – 14:10 Lunch
     
    14:10 – 16:10 Practice I – Biochemical Assays

    Module 1 – Protease assay development and validation - Dr. Sheraz Gul – Group E and F- Room 09
    LNBio
    Module 2 – High throughput binding screening using MicroScale Thermophoresis - Nanotemper – Group
    G and H– Room 22 LNBio
    Module 3 – Using LabWare LIMS/ELN to catalogue Chemical Structures & ELN (Electronic Laboratory
    Notebook) Workshop - Labware – Group A and B – Room 69 LNBio
    Module 4 – Fluorescence intensity assay for Trypanosoma brucei viability using CLARIOStar – Photonics/
    Dr. Bruno Pascoalino – Group C and D – Room 42 LNBio

    16:10 – 16:30 Coffee-break
     
    16:30 – 18:30 Practice I – Biochemical Assays
     
    Module 1 – Protease assay development and validation - Dr. Sheraz Gul – Group G and H- Room 09
    LNBio
    Module 2 – High throughput binding screening using MicroScale Thermophoresis - Nanotemper – Group
    A and B– Room 22 LNBio
    Module 3 – Using LabWare LIMS/ELN to catalogue Chemical Structures & ELN (Electronic Laboratory
    Notebook) Workshop - Labware – Group C and D – Room 69 LNBio
    Module 4 – Fluorescence intensity assay for Trypanosoma brucei viability using CLARIOStar – Photonics/
    Dr. Bruno Pascoalino – Group E and F – Room 42 LNBio

    18:30 – 20:30 Poster session II/ Pastel evening
    Supported by DNDi
     
    June, 18th
    08:30 – 09:15 Screening Databases, Hit Clustering and Prioritization
    Dr. Jean-Robert Ioset – DNDi/ Switzerland
     
    09:15 – 10:00 Host-Parasite Interactions at the Cellular Level
    Prof. Renato Mortara - UNIFESP/ Brazil
     
    10:00 - 10:45 Genetic Diversity of Kinetoplastids
    Prof. Bianca Zingales - USP/ Brazil
     
    10:45 – 11:15 Coffee-break
     
    11:15 – 12:00 Secondary Assays for Hit-to-Lead and Lead Prioritization
    Dr. Carolina B. Moraes – LNBio/ Brazil
     
    12:00 – 12:45 Recent advances in HCS technologies
    Dr. Kamyar Hadian - Helmholtz Zentrum München/ Germany
     
    13:00 – 14:10 Lunch
     
    14:10 – 16:10 Practice II – HCS/ Data Analysis
     
    Module 1 – High Content Screening in Neglected Diseases Research: the Basics – Dr.Kaliandra de
    Almeida Gonçalves/ Dr. Carolina Borsoi – Group A and B – Room 09 LNBio
    Module 2 – Phenotypic Antiparasitic Screening Data Analysis and Visualization – Dr. Sheraz Gul/ Dr.
    Kamyar Hadian – Group C and D – Room 69 LNBio
    Module 3 – Tools for HTS Data Analysis and Visualization - Dr. Jair L. Siqueira-Neto - Group E and F–
    Room 201 LNLS
    Module 4 – Screening Hit Analysis and Clustering/Screening Data Sharing – Dr. Jean-Robert Ioset –
    Group G and H – Room 227 B CTBE 
     
    16:10 – 16:30 Coffee-break
     
    16:30 – 18:30 Practice II – HCS/ Data Analysis
     
    Module 1 –High Content Screening in Neglected Diseases Research: the Basics – Dr. Kaliandra de
    Almeida Gonçalves/ Dr. Carolina Borsoi - Group C and D – Room 09 LNBio
    Module 2 – Phenotypic Antiparasitic Screening Data Analysis and Visualization – Dr. Sheraz Gul/ Dr.
    Kamyar Hadian – Group E and F – Room 69 LNBio
    Module 3 – Tools for HTS Data Analysis and Visualization - Dr. Jair L. Siqueira-Neto - Group G and H–
    Room 201 LNLS
    Module 4 – Screening Hit Analysis and Clustering/Screening Data Sharing – Dr. Jean-Robert Ioset –
    Group A and B – Room 227 B CTBE

    June, 19th

    08:30 – 09:15 Medicinal Chemistry: An Introduction
    Dr. Tom Von Geldern/ USA
     
    09:15 – 10:00 Assessing Physicochemical and ADME Properties in Early Drug Discovery
    Prof. Susan Charman –Monash Univ/ Australia
     
    10:00 - 10:45 Issues Related to Drug Development in Kinetoplastid Diseases
    Prof. Simon Croft – LSHTM/UK

    10:45 – 11:15 Coffee-break
     
    11:15 – 12:00 Pharmacokinetics Properties to Ensure Efficacy and Safety
    Prof. Susan Charman –Monash Univ/ Australia
     
    12:00 – 12:45 Compound Safety Profiling: in silico, in vitro and in vivo
    Dr. Tom Von Geldern/ USA

    13:00 – 14:10 Lunch
     
    14:10 – 16:10 Practice II – HCS/ Data Analysis
     
    Module 1 – High Content Screening in Neglected Diseases Research: the Basics – Dr. Kaliandra de
    Almeida Gonçalves/ Dr. Carolina Borsoi – Group E and F– Room 09 LNBio
    Module 2 – Phenotypic Antiparasitic Screening Data Analysis and Visualization – Dr. Sheraz Gul/ Dr.
    Kamyar Hadian – Group G, H, A and B – Room 69 LNBio
    Module 3
    Module 4 – Screening Hit Analysis and Clustering/Screening Data Sharing – Dr. Jean-Robert Ioset –
    Group C and D – Room 227 B CTBE
     
    16:10 – 16:30 Coffee-break
     
    16:30 – 18:30 Practice II – HCS/ Data Analysis
     
    Module 1 – High Content Screening in Neglected Diseases Research: the Basics – Dr. Kaliandra de
    Almeida Gonçalves/ Dr. Carolina Borsoi – Group G and H– Room 09 LNBio
    Module 2
    Module 3 - Tools for HTS Data Analysis and Visualization - Dr. Jair L. Siqueira-Neto - Group A, B, C and
    D– Room 69 LNBio
    Module 4 - Screening Hit Analysis and Clustering/Screening Data Sharing – Dr. Jean-Robert Ioset –
    Group E and F – Room 227 B CTBE
     
    18:30 – 20:30 Poster session III/ Pizza evening
    Supported by DNDi
     
    June, 20th

    08:30 – 09:15 Think Medicinal Chemistry
    Dr. Gilles Courtemanche– Bioaster/ France
     
    09:15 – 10:00 Lead Optimization Program for Chagas disease: An Example
    Martine Keenan – Epichem/ Australia
     
    10:00 - 10:45 Building a National Consortium on Neglected Diseases Drug Discovery
    Dr. Lucio Freitas-Junior – LNBio/ Brazil
     
    10:45 – 11:15 Coffee-break
     
    11:15 – 11:50 Synthesis and Biological Activity of Natural Products and Derivatives
    Prof. Dr. Ronaldo Pilli - UNICAMP/ Brazil
     
    11:50 – 12:25 New Synthetic Tools for the Construction of Molecules of Medicinal Value
    Prof. Dr. Carlos R.D. Correia – UNICAMP/ Brazil
     
    12:25 – 14:00 Lunch
     
    14:00 – 14:35 Fluorescent Tags and Probes: Mechanisms of Actions and Dynamics in Live Cells of Bioactive
    Small Molecules
    Dr. Brenno Neto - UNB/ Brazil
     
    14:35 – 15:10 Hypervalent Iodine in Synthetic Organic Chemistry
    Dr. Luiz Fernando Silva Jr-USP/Brazil
     
    15:10 – 15:45 Developing a library of heterocycles to fight neglected diseases
    Dr. Flavio da Silva Emery- USP/Brazil
     
    15:45 -16:15 Coffee-break
     
    16:15 – 17:15 Round-table
     
    17:30 – 22:00 Social gathering: Festa Junina (Typical Brazilian Festival)
     
    June, 21th

    08:00 – 10:00 Practice III: In vitro and In vivo ADME/Medicinal Chemistry Problem Solving
     
    Module 1 – Hit to Lead - Dr. Gilles Courtemanche Group A and B – Room 111 B CTBE
    Module 2 –- Lead Optimization – Dr. Martine Keenan/ Dr. Tom Von Geldern -Group C and D– Room 227
    B CTBE
    Module 3 – In vitro metabolic stability and metabolic profile – Daniel Lebre – Group E and F– Room 22
    LNBio
    Module 4 – Drug Candidates: Bioanalytical Validation and In vivo Pharmacokinetic Studies. Dr. Silvana
    A. Rocco/Raphael Morales - Group G and H– Room 69 LNBio
     
    10:00 – 10:15 Coffee-break
     
    10:15 – 12:15 Practice III: In vitro and In vivo ADME/Medicinal Chemistry Problem Solving
     
    Module 1 – Hit to Lead - Dr. Gilles Courtemanche Group C and D– Room 111 B CTBE
    Module 2 – Lead Optimization – Dr. Martine Keenan/ Dr. Tom Von Geldern – Group E and F– Room 227
    B CTBE
    Module 3 – In vitro metabolic stability and metabolic profile – Daniel Lebre - Group G and H– Room 22
    LNBio
    Module 4 – Drug Candidates: Bioanalytical Validation and In vivo Pharmacokinetic Studies.- Dr. Silvana
    A. Rocco/Raphael Morales – Group A and B– Room 69 LNBio
     
    12:15 – 13:45 Lunch
     
    13:45 – 15:45 Practice III: In vitro and In vivo ADME/Medicinal Chemistry Problem Solving
     
    Module 1 – Hit to Lead - Dr. Gilles Courtemanche – Group E and F– Room 111 B CTBE
    Module 2 – Lead Optimization – Dr. Martine Keenan/ Dr. Tom Von Geldern – Group G and H– Room 227
    B CTBE
    Module 3 – In vitro metabolic stability and metabolic profile – Daniel Lebre – Group A and B– Room 22
    LNBio
    Module 4 – Drug Candidates: Bioanalytical Validation and In vivo Pharmacokinetic Studies.- Dr. Silvana
    A. Rocco/Raphael Morales – Group C and D– Room 69 LNBio
     
    15:45 – 16:15 Coffee-break
     
    16:15 – 18:15 Practice III: In vitro and In vivo ADME/Medicinal Chemistry Problem Solving
     
    Module 1 – Hit to Lead - Dr. Gilles Courtemanche - Group G and H– Room 111 B CTBE
    Module 2 – Lead Optimization – Dr. Martine Keenan/ Dr. Tom Von Geldern – Group A and B– Room 227
    B CTBE
    Module 3 – In vitro metabolic stability and metabolic profile – Daniel Lebre – Group C and D– Room 22
    LNBio
    Module 4 – Drug Candidates: Bioanalytical Validation and In vivo Pharmacokinetic Studies.- Dr. Silvana
    A. Rocco/Raphael Morales – Group E and F– Room 69 LNBio
     
    June, 22th

    08:30 – 09:15 Drug Efficacy Models for Chagas Disease
    Prof. John Kelly – LSHTM/UK
     
    09:15 – 10:00 Animal Models of Leishmaniasis Applied to Drug Discovery
    Prof. Silvia Uliana - USP/ Brazil
     
    10:00 - 10:45 Drug Efficacy Models for Leishmaniasis and HAT
    Prof. Joana Tavares – Universidade do Porto/ Portugal
     
    10:45 – 11:15 Coffee-break
     
    11:15 – 12:00 Pre-clinical Pharmacology: How to Take a Lead Compound to Clinical Studies
    Prof. João Batista Calixto - UFSC/ Brazil
     
    12:00 –12:45 Virulence Factors in Visceral Leishmaniasis
    Prof. Carlos Henrique Nery Costa - UFPI/ Brazil
     
    13:00 – 14:10 Lunch
     
    14:10 –16:10 Practice IV: Animal models
     
    Module 1 – Chagas Disease: from bench to trench – Dr. Ana Torrecilhas – Group A and B – Room 09
    LNBio
    Module 2 – VL hamster model for Drug Efficacy – Dr. Andre Tempone - Group C and D - Room 22 LNBio
    Module 3 – Bioluminescence imaging of Kinetoplastid infections – Dr. John Kelly/Dr. Joana Tavares –
    Group E and F - Room 09 LNBio
    Module 4 – Guided tour – Group G and H – LNBio entrance
     
    16:10 –16:30 Coffee-break
     
    16:30 –18:30 Practice IV: Animal models
     
    Module 1 – Chagas Disease: from bench to trench – Dr. Ana Torrecilhas - Group C and D– Room 09
    LNBio
    Module 2 – VL hamster model for Drug Efficacy – Dr. Andre Tempone – Group E and F - Room 22 LNBio
    Module 3 – Bioluminescence imaging of Kinetoplastid infections – Dr. John Kelly/Dr. Joana Tavares –
    Group G and H - Room 09 LNBio
    Module 4 – Guided tour – Group A and B– LNBio entrance
     
    June, 23th

    08:30 – 12:45 CLOSING SESSION: Brazilian Pharma: how it can actively drive neglected diseases drug
    discovery?
    Chair: Prof. João Batista Calixto – UFSC/ Brazil
     
    08:30 – 08:45 Prof. João Batista Calixto– UFSC/ Brazil
     
    08:45 – 09:15 Dr. Kleber Franchini – Brazilian Biosciences National Laboratory (LNBio) / Brazil
     
    09:15 - 09:45 André Berto Gimenez – Brazilian Innovation Agency (FINEP) / Brazil
     
    09:45 - 10:15 Pedro Palmeira – Brazilian Development Bank (BNDES) / Brazil

    10:15 - 10:45 Simone Godoi – São Paulo Research Foundation (FAPESP) / Brazil
     
    10:45 – 11:15 Coffee-break
     
    11:15 – 11:45 Cristalia: A History of Research, Development and Innovation
    Prof. Dr. Spartaco Astolfi Filho – Cristalia/ Brazil
     
    11:45 – 12:15 Eric Chatelain - DNDi/ Switzerland
     
    12:15 – 12:45 Round table
     
    13:00 – 14:10 Lunch
     
    14:10-16:10 Practice IV: Animal models
     
    Module 1 – Chagas Disease: from bench to trench – Dr. Ana Torrecilhas – Group E and F– Room 09
    LNBio
    Module 2 – VL hamster model for Drug Efficacy – Dr. Andre Tempone – Group G and H - Room 22 LNBio
    Module 3 – Bioluminescence imaging of Kinetoplastid infections – Dr. John Kelly/Dr. Joana Tavares–
    Group A and B- Room 09 LNBio
    Module 4 – Guided tour – Group C and D– LNBio entrance
     
    16:10 – 16:30 Coffee-break
     
    16:30 – 18:30 Practice IV: Animal models
     
    Module 1 – Chagas Disease: from bench to trench – Dr. Ana Torrecilhas – Group G and H– Room 09
    LNBio
    Module 2 – VL hamster model for Drug Efficacy – Dr. Andre Tempone – Group A and B - Room 22 LNBio
    Module 3 – Bioluminescence imaging of Kinetoplastid infections – Dr. John Kelly/Dr. Joana Tavares –
    Group C and D- Room 09 LNBio
    Module 4 – Guided tour – Group E and F– LNBio entrance
     
    19:00 Closing dinner
     
    June, 24th

    09:15 – 10:40 Feedback

    10:40 – 11:00 Closing remarks