São Paulo School of Advanced Sciences on Neglected Diseases Drug Discovery – focus on Kinetoplastids

The São Paulo School of Advanced Science on Neglected Diseases Drug Discovery – focus on Kinetoplastids (SPSAS-ND3) was hosted in CNPEM - Campinas-SP, Brazil in June, 14th to 24th, 2015.

The SPSAS was funded by FAPESP (Sao Paulo Research Foundation) and aims to support courses in advanced themes in science and technology, contributing to the formation of young researchers and attraction of both national and international scientific talents to Sao Paulo state.

SPSAS-ND3 focused on multidisciplinary aspects of drug discovery applied to Chagas disease, African tripanosomiasis and leishmaniasis, introducing students to the basics of drug discovery science and also to cutting-edge research on the field.

The course was structured in both lectures and practical activities covering high throughput and high content screening, structural biology and virtual screening, screening data analysis, medicinal chemistry, lead optimization, in vitro and in vivo ADME, and in vivo models for pharmacokinetics and efficacy studies.

  • Institution

    Laboratório Nacional de Biociências / CNPEM / MCTI

  • Field of Knowledge

    Chemistry

  • Academic Director

    Lucio Holanda Gondim de Freitas Junior

  • Grant Number

    2013/50644-4

  • Date

    2015-06-14 to 2015-06-24

  • Registration Deadline

    closed

  • Site

    http://pages.cnpem.br/drugdiscovery-kinetoplastids

  • City

    Campinas

  • Keywords

    Drugs, Neglected diseases, Health, Chemistry, Biosciences

  • Program Hide
    June, 14th

    13:00 –15:00: Lunch and Registration

    15:00 –16:00: Groups division: Organizing Committee

    16:00 – 6:30: Coffee-break

    16:30 –17:30: Welcoming and General Remarks
     
    June, 15th

    08:20 – 08:45: Overview of SPSAS-ND3: dynamics and expected outcomes
    Dr. Lucio Freitas-Junior – LNBio/ Brazil

    08:45 – 09:30: Neglected Tropical Diseases: Needs and Current Drug Discovery Landscape
    Dr. Eric Chatelain – DNDi/ Switzerland

    09:30 – 10:00: Drug Discovery: The Pharmaceutical Industry Perspective
    Dr. Sheraz Gul – ScreeningPort Fraunhofer/ Germany

    10:00 – 10:45: Introduction to Drug Discovery/Design of Biochemical Assays for Drug Discovery Purposes – what can be achieved and learnings from past success and failures
    Dr. Sheraz Gul – ScreeningPort Fraunhofer/ Germany

    10:45 – 11:15: Coffee-break

    11:15 – 12:00: Identification of metabolic targets in trypanosomatids
    Dr. Artur Cordeiro - LNBio/ Brazil

    12:00 – 12:45: Virtual Screening
    Dr. Rafael Guido – USP/ Brazil

    13:00 – 14:10: Lunch
     
    14:10 – 14:40: Poster fixation
     
    14:40 – 15:30: Biosafety in level II laboratory/ Instructions for practical activities
    Dr. Carolina Borsoi / Dr. Vanessa Fontana
     
    15:30 – 16:00: Official photography
     
    16:00 – 16:50: Coffee-break
     
    16:50 – 17:10: Opening ceremony
    Dr. Kleber Franchini - LNBio/ Brazil
     
    17:10 – 17:30: Opening ceremony
    Eric Stobbaerts - DNDi Latin America
     
    17:30 – 17:50: Inaugural Class
    Prof. Michel Rabinovitch – Unifesp/Brazil
     
    17:50-19:50: Poster session I/ Opening cocktail: Brazilian evening
    Supported by DNDi
     
    June, 16th

    08:30 – 09:15 Screening jargon and terms
    Dr. Sheraz Gul – ScreeningPort Fraunhofer/ Germany
     
    09:15 – 10:00 HTS Assay Design
    Dr. Sheraz Gul – ScreeningPort Fraunhofer/ Germany
     
    10:00 - 10:45 Biochemistry of Kinetoplastids and Drug Discovery
    Prof. Sergio Schenkman - UNIFESP/ Brazil
     
    10:45 – 11:15 Coffee-break
     
    11:15 – 12:00 Drug targets in Trypanosomes
    Prof. Joana Tavares – Universidade do Porto/ Portugal
     
    12:00 – 12:45 Current Chemotherapy for leishmaniasis: Drug Mechanism of Action
    Prof. Simon Croft - LSHTM/UK
     
    13:00 – 14:10 Lunch

    14:10 – 16:10 Practice I – Biochemical Assays

    Module 1 – Protease assay development and validation- Dr. Sheraz Gul – Group A and B – Room 09
    LNBio
    Module 2 – High throughput binding screening using MicroScale Thermophoresis - Nanotemper – Group
    C and D –Room 22 LNBio
    Module 3 – Using LabWare LIMS/ELN to catalogue Chemical Structures & ELN (Electronic Laboratory
    Notebook) Workshop - LabWare – Group E and F – Room 69 LNBio
    Module 4 – Fluorescence intensity assay for Trypanosoma brucei viability using CLARIOStar – Photonics/
    Dr. Bruno Pascoalino – Group G and H – Room 42 LNBio
     
    16:10 – 16:30 Coffee-break
     
    16:30 – 18:30 Practice I – Biochemical Assays
     
    Module 1 – Protease assay development and validation - Dr. Sheraz Gul – Group C and D - Room 09
    LNBio
    Module 2 – High throughput binding screening using MicroScale Thermophoresis - Nanotemper – Group
    E and F– Room 22 LNBio
    Module 3 – Using LabWare LIMS/ELN to catalogue Chemical Structures & ELN (Electronic Laboratory
    Notebook) Workshop - LabWare – Group G and H – Room 69 LNBio
    Module 4 – Fluorescence intensity assay for Trypanosoma brucei viability using CLARIOStar – Photonics/
    Dr. Bruno Pascoalino – Group A and B – Room 42 LNBio

    June, 17th

    08:30 – 09:15 The Path to a Candidate: A multidisciplinary Effort and a Lot of Hurdles
    Dr. Eric Chatelain - DNDi/ Switzerland
     
    09:15 – 10:00 High Throughput and High Content Screening for Trypanosomes
    Prof. Vicky M Avery - Eskitis Institute/Griffith University/ Australia
     
    10:00 - 10:45 MiRNA screening for drug discovery
    Dr. Musa Mhlanga – University of Cape Town & CSIR & IMM/ South Africa
     
    10:45 – 11:15 Coffee-break
     
    11:15 – 12:00 Integrative Drug Discovery Programs
    Dr. Sheraz Gul – ScreeningPort Fraunhofer/ Germany
     
    12:00 – 12:45 HCS Data Analysis and Reduction
    Dr. Kamyar Hadian - Helmholtz Zentrum München/ Germany
     
    13:00 – 14:10 Lunch
     
    14:10 – 16:10 Practice I – Biochemical Assays

    Module 1 – Protease assay development and validation - Dr. Sheraz Gul – Group E and F- Room 09
    LNBio
    Module 2 – High throughput binding screening using MicroScale Thermophoresis - Nanotemper – Group
    G and H– Room 22 LNBio
    Module 3 – Using LabWare LIMS/ELN to catalogue Chemical Structures & ELN (Electronic Laboratory
    Notebook) Workshop - Labware – Group A and B – Room 69 LNBio
    Module 4 – Fluorescence intensity assay for Trypanosoma brucei viability using CLARIOStar – Photonics/
    Dr. Bruno Pascoalino – Group C and D – Room 42 LNBio

    16:10 – 16:30 Coffee-break
     
    16:30 – 18:30 Practice I – Biochemical Assays
     
    Module 1 – Protease assay development and validation - Dr. Sheraz Gul – Group G and H- Room 09
    LNBio
    Module 2 – High throughput binding screening using MicroScale Thermophoresis - Nanotemper – Group
    A and B– Room 22 LNBio
    Module 3 – Using LabWare LIMS/ELN to catalogue Chemical Structures & ELN (Electronic Laboratory
    Notebook) Workshop - Labware – Group C and D – Room 69 LNBio
    Module 4 – Fluorescence intensity assay for Trypanosoma brucei viability using CLARIOStar – Photonics/
    Dr. Bruno Pascoalino – Group E and F – Room 42 LNBio

    18:30 – 20:30 Poster session II/ Pastel evening
    Supported by DNDi
     
    June, 18th
    08:30 – 09:15 Screening Databases, Hit Clustering and Prioritization
    Dr. Jean-Robert Ioset – DNDi/ Switzerland
     
    09:15 – 10:00 Host-Parasite Interactions at the Cellular Level
    Prof. Renato Mortara - UNIFESP/ Brazil
     
    10:00 - 10:45 Genetic Diversity of Kinetoplastids
    Prof. Bianca Zingales - USP/ Brazil
     
    10:45 – 11:15 Coffee-break
     
    11:15 – 12:00 Secondary Assays for Hit-to-Lead and Lead Prioritization
    Dr. Carolina B. Moraes – LNBio/ Brazil
     
    12:00 – 12:45 Recent advances in HCS technologies
    Dr. Kamyar Hadian - Helmholtz Zentrum München/ Germany
     
    13:00 – 14:10 Lunch
     
    14:10 – 16:10 Practice II – HCS/ Data Analysis
     
    Module 1 – High Content Screening in Neglected Diseases Research: the Basics – Dr.Kaliandra de
    Almeida Gonçalves/ Dr. Carolina Borsoi – Group A and B – Room 09 LNBio
    Module 2 – Phenotypic Antiparasitic Screening Data Analysis and Visualization – Dr. Sheraz Gul/ Dr.
    Kamyar Hadian – Group C and D – Room 69 LNBio
    Module 3 – Tools for HTS Data Analysis and Visualization - Dr. Jair L. Siqueira-Neto - Group E and F–
    Room 201 LNLS
    Module 4 – Screening Hit Analysis and Clustering/Screening Data Sharing – Dr. Jean-Robert Ioset –
    Group G and H – Room 227 B CTBE 
     
    16:10 – 16:30 Coffee-break
     
    16:30 – 18:30 Practice II – HCS/ Data Analysis
     
    Module 1 –High Content Screening in Neglected Diseases Research: the Basics – Dr. Kaliandra de
    Almeida Gonçalves/ Dr. Carolina Borsoi - Group C and D – Room 09 LNBio
    Module 2 – Phenotypic Antiparasitic Screening Data Analysis and Visualization – Dr. Sheraz Gul/ Dr.
    Kamyar Hadian – Group E and F – Room 69 LNBio
    Module 3 – Tools for HTS Data Analysis and Visualization - Dr. Jair L. Siqueira-Neto - Group G and H–
    Room 201 LNLS
    Module 4 – Screening Hit Analysis and Clustering/Screening Data Sharing – Dr. Jean-Robert Ioset –
    Group A and B – Room 227 B CTBE

    June, 19th

    08:30 – 09:15 Medicinal Chemistry: An Introduction
    Dr. Tom Von Geldern/ USA
     
    09:15 – 10:00 Assessing Physicochemical and ADME Properties in Early Drug Discovery
    Prof. Susan Charman –Monash Univ/ Australia
     
    10:00 - 10:45 Issues Related to Drug Development in Kinetoplastid Diseases
    Prof. Simon Croft – LSHTM/UK

    10:45 – 11:15 Coffee-break
     
    11:15 – 12:00 Pharmacokinetics Properties to Ensure Efficacy and Safety
    Prof. Susan Charman –Monash Univ/ Australia
     
    12:00 – 12:45 Compound Safety Profiling: in silico, in vitro and in vivo
    Dr. Tom Von Geldern/ USA

    13:00 – 14:10 Lunch
     
    14:10 – 16:10 Practice II – HCS/ Data Analysis
     
    Module 1 – High Content Screening in Neglected Diseases Research: the Basics – Dr. Kaliandra de
    Almeida Gonçalves/ Dr. Carolina Borsoi – Group E and F– Room 09 LNBio
    Module 2 – Phenotypic Antiparasitic Screening Data Analysis and Visualization – Dr. Sheraz Gul/ Dr.
    Kamyar Hadian – Group G, H, A and B – Room 69 LNBio
    Module 3
    Module 4 – Screening Hit Analysis and Clustering/Screening Data Sharing – Dr. Jean-Robert Ioset –
    Group C and D – Room 227 B CTBE
     
    16:10 – 16:30 Coffee-break
     
    16:30 – 18:30 Practice II – HCS/ Data Analysis
     
    Module 1 – High Content Screening in Neglected Diseases Research: the Basics – Dr. Kaliandra de
    Almeida Gonçalves/ Dr. Carolina Borsoi – Group G and H– Room 09 LNBio
    Module 2
    Module 3 - Tools for HTS Data Analysis and Visualization - Dr. Jair L. Siqueira-Neto - Group A, B, C and
    D– Room 69 LNBio
    Module 4 - Screening Hit Analysis and Clustering/Screening Data Sharing – Dr. Jean-Robert Ioset –
    Group E and F – Room 227 B CTBE
     
    18:30 – 20:30 Poster session III/ Pizza evening
    Supported by DNDi
     
    June, 20th

    08:30 – 09:15 Think Medicinal Chemistry
    Dr. Gilles Courtemanche– Bioaster/ France
     
    09:15 – 10:00 Lead Optimization Program for Chagas disease: An Example
    Martine Keenan – Epichem/ Australia
     
    10:00 - 10:45 Building a National Consortium on Neglected Diseases Drug Discovery
    Dr. Lucio Freitas-Junior – LNBio/ Brazil
     
    10:45 – 11:15 Coffee-break
     
    11:15 – 11:50 Synthesis and Biological Activity of Natural Products and Derivatives
    Prof. Dr. Ronaldo Pilli - UNICAMP/ Brazil
     
    11:50 – 12:25 New Synthetic Tools for the Construction of Molecules of Medicinal Value
    Prof. Dr. Carlos R.D. Correia – UNICAMP/ Brazil
     
    12:25 – 14:00 Lunch
     
    14:00 – 14:35 Fluorescent Tags and Probes: Mechanisms of Actions and Dynamics in Live Cells of Bioactive
    Small Molecules
    Dr. Brenno Neto - UNB/ Brazil
     
    14:35 – 15:10 Hypervalent Iodine in Synthetic Organic Chemistry
    Dr. Luiz Fernando Silva Jr-USP/Brazil
     
    15:10 – 15:45 Developing a library of heterocycles to fight neglected diseases
    Dr. Flavio da Silva Emery- USP/Brazil
     
    15:45 -16:15 Coffee-break
     
    16:15 – 17:15 Round-table
     
    17:30 – 22:00 Social gathering: Festa Junina (Typical Brazilian Festival)
     
    June, 21th

    08:00 – 10:00 Practice III: In vitro and In vivo ADME/Medicinal Chemistry Problem Solving
     
    Module 1 – Hit to Lead - Dr. Gilles Courtemanche Group A and B – Room 111 B CTBE
    Module 2 –- Lead Optimization – Dr. Martine Keenan/ Dr. Tom Von Geldern -Group C and D– Room 227
    B CTBE
    Module 3 – In vitro metabolic stability and metabolic profile – Daniel Lebre – Group E and F– Room 22
    LNBio
    Module 4 – Drug Candidates: Bioanalytical Validation and In vivo Pharmacokinetic Studies. Dr. Silvana
    A. Rocco/Raphael Morales - Group G and H– Room 69 LNBio
     
    10:00 – 10:15 Coffee-break
     
    10:15 – 12:15 Practice III: In vitro and In vivo ADME/Medicinal Chemistry Problem Solving
     
    Module 1 – Hit to Lead - Dr. Gilles Courtemanche Group C and D– Room 111 B CTBE
    Module 2 – Lead Optimization – Dr. Martine Keenan/ Dr. Tom Von Geldern – Group E and F– Room 227
    B CTBE
    Module 3 – In vitro metabolic stability and metabolic profile – Daniel Lebre - Group G and H– Room 22
    LNBio
    Module 4 – Drug Candidates: Bioanalytical Validation and In vivo Pharmacokinetic Studies.- Dr. Silvana
    A. Rocco/Raphael Morales – Group A and B– Room 69 LNBio
     
    12:15 – 13:45 Lunch
     
    13:45 – 15:45 Practice III: In vitro and In vivo ADME/Medicinal Chemistry Problem Solving
     
    Module 1 – Hit to Lead - Dr. Gilles Courtemanche – Group E and F– Room 111 B CTBE
    Module 2 – Lead Optimization – Dr. Martine Keenan/ Dr. Tom Von Geldern – Group G and H– Room 227
    B CTBE
    Module 3 – In vitro metabolic stability and metabolic profile – Daniel Lebre – Group A and B– Room 22
    LNBio
    Module 4 – Drug Candidates: Bioanalytical Validation and In vivo Pharmacokinetic Studies.- Dr. Silvana
    A. Rocco/Raphael Morales – Group C and D– Room 69 LNBio
     
    15:45 – 16:15 Coffee-break
     
    16:15 – 18:15 Practice III: In vitro and In vivo ADME/Medicinal Chemistry Problem Solving
     
    Module 1 – Hit to Lead - Dr. Gilles Courtemanche - Group G and H– Room 111 B CTBE
    Module 2 – Lead Optimization – Dr. Martine Keenan/ Dr. Tom Von Geldern – Group A and B– Room 227
    B CTBE
    Module 3 – In vitro metabolic stability and metabolic profile – Daniel Lebre – Group C and D– Room 22
    LNBio
    Module 4 – Drug Candidates: Bioanalytical Validation and In vivo Pharmacokinetic Studies.- Dr. Silvana
    A. Rocco/Raphael Morales – Group E and F– Room 69 LNBio
     
    June, 22th

    08:30 – 09:15 Drug Efficacy Models for Chagas Disease
    Prof. John Kelly – LSHTM/UK
     
    09:15 – 10:00 Animal Models of Leishmaniasis Applied to Drug Discovery
    Prof. Silvia Uliana - USP/ Brazil
     
    10:00 - 10:45 Drug Efficacy Models for Leishmaniasis and HAT
    Prof. Joana Tavares – Universidade do Porto/ Portugal
     
    10:45 – 11:15 Coffee-break
     
    11:15 – 12:00 Pre-clinical Pharmacology: How to Take a Lead Compound to Clinical Studies
    Prof. João Batista Calixto - UFSC/ Brazil
     
    12:00 –12:45 Virulence Factors in Visceral Leishmaniasis
    Prof. Carlos Henrique Nery Costa - UFPI/ Brazil
     
    13:00 – 14:10 Lunch
     
    14:10 –16:10 Practice IV: Animal models
     
    Module 1 – Chagas Disease: from bench to trench – Dr. Ana Torrecilhas – Group A and B – Room 09
    LNBio
    Module 2 – VL hamster model for Drug Efficacy – Dr. Andre Tempone - Group C and D - Room 22 LNBio
    Module 3 – Bioluminescence imaging of Kinetoplastid infections – Dr. John Kelly/Dr. Joana Tavares –
    Group E and F - Room 09 LNBio
    Module 4 – Guided tour – Group G and H – LNBio entrance
     
    16:10 –16:30 Coffee-break
     
    16:30 –18:30 Practice IV: Animal models
     
    Module 1 – Chagas Disease: from bench to trench – Dr. Ana Torrecilhas - Group C and D– Room 09
    LNBio
    Module 2 – VL hamster model for Drug Efficacy – Dr. Andre Tempone – Group E and F - Room 22 LNBio
    Module 3 – Bioluminescence imaging of Kinetoplastid infections – Dr. John Kelly/Dr. Joana Tavares –
    Group G and H - Room 09 LNBio
    Module 4 – Guided tour – Group A and B– LNBio entrance
     
    June, 23th

    08:30 – 12:45 CLOSING SESSION: Brazilian Pharma: how it can actively drive neglected diseases drug
    discovery?
    Chair: Prof. João Batista Calixto – UFSC/ Brazil
     
    08:30 – 08:45 Prof. João Batista Calixto– UFSC/ Brazil
     
    08:45 – 09:15 Dr. Kleber Franchini – Brazilian Biosciences National Laboratory (LNBio) / Brazil
     
    09:15 - 09:45 André Berto Gimenez – Brazilian Innovation Agency (FINEP) / Brazil
     
    09:45 - 10:15 Pedro Palmeira – Brazilian Development Bank (BNDES) / Brazil

    10:15 - 10:45 Simone Godoi – São Paulo Research Foundation (FAPESP) / Brazil
     
    10:45 – 11:15 Coffee-break
     
    11:15 – 11:45 Cristalia: A History of Research, Development and Innovation
    Prof. Dr. Spartaco Astolfi Filho – Cristalia/ Brazil
     
    11:45 – 12:15 Eric Chatelain - DNDi/ Switzerland
     
    12:15 – 12:45 Round table
     
    13:00 – 14:10 Lunch
     
    14:10-16:10 Practice IV: Animal models
     
    Module 1 – Chagas Disease: from bench to trench – Dr. Ana Torrecilhas – Group E and F– Room 09
    LNBio
    Module 2 – VL hamster model for Drug Efficacy – Dr. Andre Tempone – Group G and H - Room 22 LNBio
    Module 3 – Bioluminescence imaging of Kinetoplastid infections – Dr. John Kelly/Dr. Joana Tavares–
    Group A and B- Room 09 LNBio
    Module 4 – Guided tour – Group C and D– LNBio entrance
     
    16:10 – 16:30 Coffee-break
     
    16:30 – 18:30 Practice IV: Animal models
     
    Module 1 – Chagas Disease: from bench to trench – Dr. Ana Torrecilhas – Group G and H– Room 09
    LNBio
    Module 2 – VL hamster model for Drug Efficacy – Dr. Andre Tempone – Group A and B - Room 22 LNBio
    Module 3 – Bioluminescence imaging of Kinetoplastid infections – Dr. John Kelly/Dr. Joana Tavares –
    Group C and D- Room 09 LNBio
    Module 4 – Guided tour – Group E and F– LNBio entrance
     
    19:00 Closing dinner
     
    June, 24th

    09:15 – 10:40 Feedback

    10:40 – 11:00 Closing remarks 

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